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Data that’s available contains informati… The neural cell adhesion molecule (N-CAM) inhibits proliferation in main cultures of rat astrocytes. The mechanism of binding of neural cell adhesion molecules.
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- These outcomes point out that in cultured neural tissue, the E type of N-CAM isn’t processed into A forms but is steadily degraded and changed by newly synthesized A forms.
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The fee of E-to-A conversion and the proportions of the totally different A varieties synthesized in vitro have been much like these noticed for the tissues in vivo at comparable times. These results counsel that the submit translational processing in vitro was similar to that in vivo. The knowledge had been consistent with the possibility that the rise in synthesis of A forms occurred both in cells that had beforehand synthesized E varieties or in a definite population of cells that already synthesized A types. In pulse-chase experiments, the E type of N-CAM that was synthesized during the first day after explantation persisted as E type for several days, at instances when newly synthesized N-CAM was predominantly in A types.
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Patients And Methods
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During normal development, the neural cell adhesion molecule N-CAM modifications on the cell-surface from a sialic acid-rich embryonic, or E kind, to several adult, or A types which have less sialic acid (E-to-A conversion). To investigate the mobile and molecular mechanisms that underlie these adjustments, we’ve established situations under which E-to-A conversion happens in cultured explants of central nervous system tissues. Mouse cerebellum, chick spinal wire, and chick retina that express the E form of N-CAM were dissected and cultured on collagen gels. After 3-6 d in culture, increased proportions of A types have been synthesized, as revealed by specific immunoprecipitation and immunoblotting.
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